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The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.

Features:

• 1）The highest yields of high-quality sequencing libraries from single cells, or 2 pg - 200 ng RNA
• 2）Input can be cultured or primary cells, or total RNA
• 3）Low abundance transcripts are easily detected
• 5）High quality libraries and sequence data are generated for a wide range input amounts and sample types
  •         Full length transcript coverage
  •         Consistent transcript detection
  •         Superior transcript correlation
• 6）Fast, streamlined, automation-friendly workflow, with minimal handling steps and hands-on time
  •         Single-tube protocol from cell lysis to cDNA
  •         DNA fragmentation, end repair and dA-tailing reagents in a single enzyme mix, with a single protocol, regardless of GC content