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Recombinant Human Transforming Growth Factor Beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.

分子量

12.8 KDa

表观分子量

13 KDa, reducing conditions

剂型描述

Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mM NaCl, pH 2.5.

内毒素

< 0.01 EU/µg as determined by LAL test.

纯度-SDS-PAGE

Greater than 95% as determined by reducing SDS-PAGE.

生物活性-Cell Based Assay

Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml (QC verified).

复溶

• Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
• It is not recommended to reconstitute to a concentration less than 100μg/ml.
• Dissolve the lyophilized protein in distilled water.
• Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

运输条件

• The product is shipped at ambient temperature.
• Upon receipt, store it immediately at the temperature listed below.

保存条件

• Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

产品背景

Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.