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Recombinant Human Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn971-Arg1023 is expressed.

蛋白编号

P01133

分子量

6.2 KDa

表观分子量

11 KDa, reducing conditions

剂型描述

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.0.

内毒素

< 0.01 EU/µg as determined by LAL test.

纯度-SDS-PAGE

Greater than 95% as determined by reducing SDS-PAGE.

生物活性-Cell Based Assay

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 60-450 pg/ml (QC verified).

Mouse intestinal organoids were cultured with EGF (C029), Noggin (C028) and R-spondin 1 (CX83). After five days of culture, the organoids showed good morphology and germination rate.

Mouse Colon organoids were cultured with EGF (C029), Wnt3a (C22R), Noggin (C028) and R-spondin 1 (CX83). The organoids showed good morphology.

Mouse Gastric organoids were cultured with EGF (C029), Wnt3a (C22R), Noggin (C028) , R-spondin 1 (CX83) and FGF-10 (CR11). The organoids showed good morphology.

Mouse bile duct organoids were cultured with EGF (C029) , R-spondin 1 (CX83) ,FGF-10 (CR11) and HGF (CJ72). The organoids showed good morphology.

Human intestinal organoids (ipsc source) were cultured with Activin A(C687) , BMP-4(CR93) , EGF (C029) , Wnt3a (C22R), Noggin (C028), R-spondin 1 (CX83) and FGF-4 (CR08) . The organoids showed good morphology.

Human breast cancer organoids were cultured with Wnt3a (C22R), Noggin (CB89) , R-spondin 3 (C18C) , KGF(CH73), FGF-10 (CR11), EGF (C029) and NRG1-beta 1 (753). The organoids showed good morphology.

复溶

• Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
• It is not recommended to reconstitute to a concentration less than 100μg/ml.
• Dissolve the lyophilized protein in distilled water.
• Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 

运输条件

• The product is shipped at ambient temperature.
• Upon receipt, store it immediately at the temperature listed below.

保存条件

• Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
• Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
• Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

产品背景

Epidermal growth factor (EGF) is a small 53 amino acid residue long protein that contains three disulfide bridges. It is a small mitogenic protein that is thought to be involved in mechanisms such as normal cell growth, oncogenesis, and wound healing. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. This protein shows both strong sequential and functional homology with human type-alpha transforming growth factor (hTGF alpha), which is a competitor for EGF receptor sites.